Breast adenocarcinoma-derived exosomes lower first-contact de-adhesion strength of adenocarcinoma cells to brain endothelial layer

• Breast adenocarcinoma- brain endothelial de-adhesion dynamics is presented. Adenocarcinoma-derived exosomes alter pattern of endothelium. Intercellular mechanics might play important role in metastatic niche formation. Despite advances modern therapeutics, one the most feared complications cancer are metastases, which often cause life impairing profound neurological symptoms and premature death. adenocarcinoma among leading “sources” metastases. Since central nervous system lacks a classical lymphatic circulation, invading cells can reach parenchyma only through haematogenous routes must breach blood-brain barrier (BBB). The key step before transmigration highly regulated interface BBB establishment firm adhesion between tumor cell cerebral layer. Using atomic force microscopy, as high resolution spectrograph, direct measurements intercellular interactions was performed living confluent layer pre-treated with carcinoma cell-derived exosomes. By immobilization to an microscope’s cantilever, de-adhesions were directly measured by single spectroscopy (SCFS) at quasi-physiological conditions. De-adhesion strength characterized several different calculated parameters, involving aspects both membrane surface related factors. Our results indicate that lower case exosome compared non-treated controls. adenocarcinoma-derived have effect on